walker a motif造句

• The proteins generally share a long Walker A motif and Walker B motif.
• This histidine contacts residues across the dimer interface in the Walker A motif and the D loop, a conserved sequence following the Walker B motif.
• The Walker A motif is best known for its presence in ATP-and GTP-binding proteins, and is also found in a variety of proteins with phosphorylated substrates.
• The A-loop ( Aromatic residue interacting with the adenine ring of ATP ) refers to conserved aromatic amino acids, essential for ATP-binding, found about 25 amino acids upstream of the Walker A motif in a subset of P-loop proteins.
• Two molecules of ATP are positioned at the interface of the dimer, sandwiched between the Walker A motif of one subunit and the LSGGQ motif of the other . and reported in structures of MJ0796, the NBD subunit of the LolD transporter from " Methanococcus jannaschii"
• The larger domain typically consists of two ?-sheets and six ? helices, where the catalytic " Walker A motif " ( GXXGXGKS / T where X is any amino acid ) or " P-loop " and " Walker B motif " ( ????D, of which ? is a hydrophobic residue ) is situated.
• Sav1866 is a homodimer of half transporters, and each subunit contains an N-terminal TMD with six helices and a C-terminal NBD . The NBDs are similar in structure to those of other ABC transporters, in which the two ATP binding sites are formed at the dimer interface between the Walker A motif of one NBD and the LSGGQ motif of the other.
• Binding of ATP is stabilized by the following interactions : ( 1 ) ring-stacking interaction of a conserved aromatic residue preceding the Walker A motif and the adenosine ring of ATP, ( 2 ) hydrogen-bonds between a conserved lysine residue in the Walker A motif and the oxygen atoms of the ?-and ?-phosphates of ATP and coordination of these phosphates and some residues in the Walker A motif with Mg 2 + ion, In addition, a residue that suggests the tight coupling of ATP binding and dimerization, is the conserved histidine in the H-loop.
• Binding of ATP is stabilized by the following interactions : ( 1 ) ring-stacking interaction of a conserved aromatic residue preceding the Walker A motif and the adenosine ring of ATP, ( 2 ) hydrogen-bonds between a conserved lysine residue in the Walker A motif and the oxygen atoms of the ?-and ?-phosphates of ATP and coordination of these phosphates and some residues in the Walker A motif with Mg 2 + ion, In addition, a residue that suggests the tight coupling of ATP binding and dimerization, is the conserved histidine in the H-loop.
• Binding of ATP is stabilized by the following interactions : ( 1 ) ring-stacking interaction of a conserved aromatic residue preceding the Walker A motif and the adenosine ring of ATP, ( 2 ) hydrogen-bonds between a conserved lysine residue in the Walker A motif and the oxygen atoms of the ?-and ?-phosphates of ATP and coordination of these phosphates and some residues in the Walker A motif with Mg 2 + ion, In addition, a residue that suggests the tight coupling of ATP binding and dimerization, is the conserved histidine in the H-loop.